Glyphosate, aminomethylphosphonic acid (AMPA), imazapyr, sulfometuron methyl (SMM), and metsulfuron methyl (MSM) were measured in streamwater collected during and after a routine application of herbicides to a forestry site in Oregon’s Coast Range. Samples were collected at three stations: HIGH at the fish/no-fish interface in the middle of the harvest/spray unit; MID at the bottom of the unit; and LOW downstream of the unit. All herbicides were applied by helicopter in a single tank mix. AMPA, imazapyr, SMM, and MSM were not detected (ND) in any sample at 15, 600, 500, and 1000 ng/L, respectively. A pulse of glyphosate peaking at ≈62 ng/L manifested at HIGH during the application. Glyphosate pulses peaking at 115 ng/L (MID) and 42 ng/L (HIGH) were found during the first two post-application storm events 8 and 10 days after treatment (DAT), respectively: glyphosate was <20 ng/L (ND) at all stations during all subsequent storm events. All glyphosate pulses were short-lived (4 to 12 h). Glyphosate in baseflow was ≈25 ng/L at all stations 3 DAT and was still ≈25 ng/L at HIGH, but ND at the other stations, 8 DAT: subsequently, glyphosate was ND in baseflow at all stations. These results show that aquatic organisms were subjected to multiple short-duration, low-concentration glyphosate pulses corresponding to a cumulative time-weighted average (TWA) exposure of 6634 ng/L*h. Comparisons to TWA exposures associated with a range of toxicological endpoints for sensitive aquatic organisms suggests a margin of safety exceeding 100 at the experimental site, with the only potential exception resulting from the ability of fish to detect glyphosate via olfaction. For imazapyr, SMM, and MSM the NDs were at concentrations low enough to rule out effects on all organisms other than aquatic plants, and the low concentration and (assumed) pulsed nature of any exposure should mitigate this potential.